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- Flow Cytometry Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 Warning: Cannot modify header information - headers already sent by (output started at /volume2/web/signalife/index.php:1) in /volume2/web/signalife/wp-includes/rest-api/class-wp-rest-server.php on line 1372 {"id":940,"date":"2013-10-02T11:05:06","date_gmt":"2013-10-02T10:05:06","guid":{"rendered":"http:\/\/signalife.unice.fr\/?page_id=940"},"modified":"2019-09-27T15:14:21","modified_gmt":"2019-09-27T14:14:21","slug":"team-research-activities","status":"publish","type":"page","link":"https:\/\/signalife.univ-cotedazur.fr\/?page_id=940","title":{"rendered":"49 Teams Research activities"},"content":{"rendered":"
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Abad Pierre – Plant nematode interactions<\/h2>\n
<\/p>\nABAD Pierre<\/h3>\n
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\n <\/td>\n <\/td>\n ABAD Pierre, INRA<\/strong> Group Leader ISA pierre.abad@sophia.inra.fr<\/a> 0492386602
\nWebsite<\/a><\/td>\n<\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Plant nematode interactions<\/strong><\/p>\n
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\n Root-knot nematodes (rkn) induce host giant cells. A. Arabidopsis GFP line & rkn larvae; B. Giant cells; C. Rkn proteins target host nuclei<\/td>\n <\/td>\n Plant-parasitic nematodes have evolved sophisticated strategies for exploiting plants. These pathogens establish a long-lasting, intimate relationship with their hosts. Interaction involves the redifferentiation of root cells into specialised multinucleate feeding cells, named giant cells that provide nutrients to the parasite. Plant parasitic nematodes inject proteins into the host to modulate both plant developmental and immune signalling pathways. Our main model system is the root-knot nematode (RKN) Meloidogyne incognita. RKN is one of the most devastating plant pathogens able to infect thousand of plant species. We coordinated the international network on the genome sequence. We develop an integrated approach on both partners of the interaction combining plant pathology, plant biotechnology, molecular genetics, comparative genomics and advanced microscopy. Our aim is to identify key players involved in signalling pathways of host\u2013parasite molecular dialogue i.e. the targets of RKN effectors in roots of the plant model Arabidopsis, the molecular mechanisms underlying the trade-off between development and immunity in plant roots and nematode adaptation to the host. Research into nematode parasitism thus tackles fundamental questions in plant development and plant pathology with the aims to design sustainable and environmentally friendly new methods to control nematodes.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Selected Publications<\/strong><\/p>\n
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\n 1.<\/td>\n A root-knot nematode-secreted protein is injected into giant cells and targeted to the nuclei Jaouannet M, Perfus-Barbeoch L, Deleury E, Magliano M, Engler G, Vieira P, Danchin EG, Da Rocha M, Coquillard P, Abad P, and Rosso MN (2012) New Phytol<\/strong> 194, 924-931<\/td>\n<\/tr>\n \n 2.<\/td>\n Multiple lateral gene transfers and duplications have promoted plant parasitism ability in nematodes Danchin EGJ, Rosso M-N, Vieira P, de Almeida-Engler J, Coutinho P, Henrissat B, Abad P (2010) Proc. <\/strong>Natl. Acad. Sci. USA.<\/strong> 107, 17651-17656<\/td>\n<\/tr>\n \n 3.<\/td>\n MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis Caillaud MC, Lecomte P, Jammes F, Quentin M, Pagnotta S, Andrio E, de Almeida Engler JD, Marfaing N, Gounon, P Abad P, and Favery B (2008) Plant Cell<\/strong> 20, 423-437<\/td>\n<\/tr>\n \n 4.<\/td>\n Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita Abad P, Gouzy J, Aury J-M, Castagnone-Sereno P, Danchin EGJ, Deleury E, Perfus-Barbeoch L et al (2008) Nature Biotech<\/strong> 26, 909-915<\/td>\n<\/tr>\n \n 5.<\/td>\n Arabidopsis formin AtFH6 is a plasma membrane\u2013associated protein upregulated in giant cells induced by parasitic nematodes Favery B, Chelysheva L, Lebris M, Jammes F, De Almeida-Engler J, Marmagne A, Lecomte P Vaury C, Arkowitz RA, Abad P (2004) Plant Cell <\/strong>16, 2529-2540<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Awards<\/strong> 2012: Prize of the Academy of Sciences \u2013 Grand prix de l\u2019Acad\u00e9mie des Sciences Roger-Jean et Chantal Gautheret \u2013 Plant Physiology & Biotechnology
<\/strong> <\/div>\n<\/li>\n- \n
Antonny Bruno – Dynamics of lipid membranes and protein coats<\/h2>\n
<\/p>\nANTONNY Bruno<\/h3>\n
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\n <\/td>\n <\/td>\n ANTONNY Bruno, CNRS<\/strong> Group Leader IPMC antonny@ipmc.cnrs.fr<\/a> 04 93 95 77 75 http:\/\/www.ipmc.cnrs.fr\/?page=antonny<\/a><\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Dynamics of lipid membranes and protein coats <\/strong><\/p>\n
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\n Studying membrane remodeling reactions using biochemical, biophysical, computer-based and cellular approaches<\/td>\n <\/td>\n Various proteins remodel the membranes of organelles involved in intracellular transport. Protein coats deform membranes to promote the budding of vesicles. Golgins, sort of molecular strings, tether vesicles to restrict their diffusion. Lipid transporters adjust the membrane composition. Although very different, most of these mechanisms are controlled by small G proteins of the Arf family and by the physical chemistry of membranes.We study these mechanisms through molecular, cellular and in silico approaches. With original assays based on fluorescence and light scattering, we follow elementary reactions such as the assembly cycle of protein coats, the tethering of liposomes by a golgin or the transfer of lipids. With fluorescence light microscopy and electron microscopy, we visualize these events in cells and in reconstituted systems. With molecular dynamics, we describe at the atomic level how specific protein motifs sense the chemistry and curvature of lipid membranes.Recent achievements: – Discovery of a general motif to sense membrane curvature: the ALPS motif – Elucidation of the mechanism by which alpha synuclein, a protein involved in Parkinson disease, recognizes the curvature of endocytic vesicles. – Discovery of a mechanism by which cholesterol is transported through the coupled transfer and hydrolysis of phosphoinositides.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Selected Publications<\/strong><\/p>\n
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\n 1.<\/td>\n Amphipathic lipid packing sensor motifs: probing bilayer defects with hydrophobic residues Vanni S, Vamparys L, Gautier R, Drin G, Etchebest C, Fuchs PF and Antonny B (2013) Biophys J<\/strong> 104, 575-584<\/td>\n<\/tr>\n \n 2.<\/td>\n Curvature, lipid packing, and electrostatics of membrane organelles: defining cellular territories in determining specificity Bigay J and Antonny B (2013) Dev Cell<\/strong> 23, 886-895<\/td>\n<\/tr>\n \n 3.<\/td>\n Osh4p exchanges sterols for phosphatidylinositol 4-phosphate between lipid bilayers de Saint-Jean M, Delfosse V, Douguet D, Chicanne G, Payrastre B, Bourguet W, Antonny B and Drin G (2011) J Cell Biol<\/strong> 195, 965-978<\/td>\n<\/tr>\n \n 4.<\/td>\n alpha-Synuclein and ALPS motifs are membrane curvature sensors whose contrasting chemistry mediates selective vesicle binding Pranke IM, Morello V, Bigay J, Gibson K, Verbavatz JM, Antonny B and Jackson CL (2011) J Cell Biol<\/strong> 194, 89-103<\/td>\n<\/tr>\n \n 5.<\/td>\n Asymmetric tethering of flat and curved lipid membranes by a golgin Drin G, Morello V, Casella JF, Gounon P and Antonny B (2008) Science<\/strong> 320, 670-673<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Awards<\/strong> 2010: CNRS bronze medal, Guillaume Drin 2010: ERC advanced grant, Bruno Antonny 2009: CNRS silver medal, Bruno Antonny 2008: EMBO member, Bruno Antonny
<\/strong> <\/div>\n<\/li>\n- \n
Arkowitz Robert – Polarized growth in yeast<\/h2>\n
<\/p>\nARKOWITZ Robert<\/h3>\n
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\n <\/td>\n <\/td>\n ARKOWITZ Robert, CNRS<\/strong> Group Leader iBV arkowitz@unice.fr<\/a> 0492076425 http:\/\/ibv.unice.fr\/EN\/equipe\/arkowitz.php<\/a><\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Polarized growth in yeast<\/strong><\/p>\n
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\n The yeast (left) to filamentous (right) transition is critical for Candida albicans<\/em> virulence. Colonies (top) & individual cells (bottom).<\/td>\n <\/td>\n Polarized growth is essential for both internal organization of cells and generation of complex multi-cellular structures. Our main interest is how cells spatially and temporally regulate their growth.Our goal is to understand the mechanisms of polarized growth and cell morphogenesis in yeast. The yeast Saccharomyces cerevisiae<\/em> reproduces during its haploid mitotic life cycle by budding. When haploid cells of the opposite mating type come in contact they direct their growth towards one another, forming pear shaped polarized cells, which ultimately fuse to form diploids. Upon nitrogen starvation diploid yeast switch from unicellular budding to a filamentous form comprised of chains of elongated cells. The opportunistic human pathogenic yeast Candida albicans<\/em> switches from an oval form to an invasive, filamentous hyphal form, a process critical for its pathogenicity.In these 3 growth processes asymmetric cell growth is accomplished by polarization of the actin cytoskeleton and subsequent localized growth by directed membrane traffic. During budding, polarized growth is initiated by internal signals whereas during mating and hyphal formation, it is dictated by external signals. We are interested in how internal and external signals are linked to site-specific growth, with particular focus on the roles of small G-proteins and phospholipids both in S. cerevisiae<\/em> and C. albicans<\/em>.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Selected Publications<\/strong><\/p>\n
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\n 1.<\/td>\n Phosphoinositides-bis-phosphate is required for Saccharomyces cerevisiae invasive growth.Guillas I, Vernay A, Vitagliano JJ and Arkowitz RA. (2013) J Cell Sci<\/strong> in press<\/td>\n<\/tr>\n \n 2.<\/td>\n A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth Vernay A, Schaub S, Guillas I, Bassilana M and Arkowitz, RA (2012) J Cell Biol<\/strong> 198, 711-730<\/td>\n<\/tr>\n \n 3.<\/td>\n Polarized growth in fungi: symmetry breaking and hyphal formation Arkowitz RA and Bassilana M (2011) Semin Cell Dev Biol <\/strong>22, 806-815<\/td>\n<\/tr>\n \n 4.<\/td>\n The Candida albicans ELMO homologue functions together with Rac1 and Dck1, upstream of the MAP Kinase Cek1, in invasive filamentous growth Hope H, Schmauch C, Arkowitz RA and Bassilana M (2010) Mol Microbiol<\/strong> 76, 1572-1590<\/td>\n<\/tr>\n \n 5.<\/td>\n Activation of Rac1 by the Guanine Nucleotide Exchange Factor Dck1 Is Required for Invasive Filamentous Growth in the Pathogen Candida albicans Hope H, Bogliolo S, Arkowitz RA and Bassilana M (2008) Mol Biol Cell<\/strong> 19, 3638-3651<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Awards<\/strong> 2013: Marie Curie ITN Consortium 2001-2010: Fondation pour la Recherche M\u00e9dicale \u2013 BNP Paribas Award 2002-2004: La Ligue Contre le Cancer \u2013 Selected Team 2001-: EMBO Young Investigator Programme 2000-2003: CNRS ATIP (start up package) <\/div>\n<\/li>\n
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Auberger Patrick – Cell Death, Differentiation, Inflammation and Cancer<\/h2>\n
<\/p>\nAUBERGER Patrick<\/h3>\n
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\n <\/td>\n <\/td>\n AUBERGER Patrick, Inserm<\/strong> Group Leader C3M auberger@unice.fr<\/a> 04 89 06 43 11 http:\/\/www.unice.fr\/c3m\/EN\/Equipe2.html<\/a><\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Cell deaths, Differentiation, Inflammation and Cancer<\/strong><\/p>\n
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\n Effect of a new triazole nucleoside (#236) on tumor formation in athymic mice. Overall survival rates in treated mice .<\/td>\n <\/td>\n Our team investigates the deregulation of cell death processes and autophagy in hematopoietic malignancies and during skin inflammatory processes. We are also developping new alternative therapeutic strategies to circumvent the resistance to conventionnal chemotherapies focusing our interest on Leukemia, Myelodysplastic Syndromes and Myeloma. We are also interested in the role of teh tyrosine kinase Lyn and of inflammatory caspases in the physiopathology of human psoriasis. In this context our mains focus of interest are: 1- The mechanisms of resistance to tyrosine kinase inhibitors in Chronic Myelogenous Leukemia (CML) 2- The mechanisms of resistance to nucleoside analogues in MyeloDysplastic Syndromes (MDS) and Acute Myeloid Leukemia (AML) 3- The role of BCL2L10 (BCL-B), an anti-apoptotic member of the BCL2 family in the homeostasis of plasma cells and the physiopathology of Multiple Myeloma (MM) 4- Role of the inflammatory caspases in the context of skin disorders such as prosiasis: pre-clinical and clinical study. 5- Regulation of the pro-apoptotic function of a bcl-2 family member by oncogenic tyrosine kinases of the Src family. Our research project is at the Biology \/ Chemistry \/ Clinical interface and aims at validating new biomarkers and drug candidates for the treatment of some hematopoietic malignancies (CML, MDS, AML, MM) and psoriasis. Keywords : Autophagy, Cell<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Selected Publications<\/strong><\/p>\n
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\n 1.<\/td>\n The caspase 6 derived N-terminal fragment of DJ-1 promotes apoptosis via increased ROS production Robert G, Puissant A, Dufies M, Marchetti S, Jacquel A, Cluzeau T, Colosetti P, Belhacene N, Kahle P, Da Costa CA, Luciano, F Checler, F and Auberger P (2012) Cell Death Differ<\/strong> 19, 1769-1778<\/td>\n<\/tr>\n \n 2.<\/td>\n Imatinib triggers mesenchymal-like conversion of CML cells associated with increased aggressiveness Puissant A, Dufies M, Fenouille N, Ben Sahra I, Jcquel A, Robert G, Cluzeau T, Deckert M, Tichet M, Cheli Y Cassuto JP, Raynaud S, Legros L, Pasquet JM, Mahon FX, Luciano F and Auberger P (2012) J Mol Cell Biol<\/strong> 4, 207-220<\/td>\n<\/tr>\n \n 3.<\/td>\n Autophagy is required for CSF-1induced macrophagic differentiation and acquisition of pahgocytic function Jacquel A, Obba S, Boyer L, Dufies M, Robert G, Gounon P, Lemichez E, Luciano F, Solary E and Auberger P (2012) Blood<\/strong> 119, 4527-4531<\/td>\n<\/tr>\n \n 4.<\/td>\n Cathepsin B release after imatinib-mediated lysosomal membrane permeabilization triggers BCR-ABL cleavage and elimination of chronic myelogenous leukemia cells Puissant A, colosetti P, Robert G, cassuto JP, Raynaud S and Auberger P (2010) Leukemia<\/strong> 24, 115-124<\/td>\n<\/tr>\n \n 5.<\/td>\n The caspase-cleaved form of LYN mediates a psoriatic-like inflammatory syndrome in mice Marchetti S, Gamas P, Belhacene N, Grosso S, Pradelli L, Colosetti P, Johansen C, Iversen L, Dekert M, Luciano P, Hofman P, Ortonne N, Khemis A Mari B, Ortonne JP, Ricci JE and Auberger P (2009) EMBO J<\/strong> 28, 2449-2460<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
<\/strong> <\/div>\n<\/li>\n- \n
Ballotti Robert – Biology and pathology of melanocytic cells: from cutaneous pigmentation to melanomas<\/h2>\n
<\/p>\nBALLOTTI Robert<\/h3>\n
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\n <\/td>\n <\/td>\n BALLOTTI Robert, Inserm<\/strong> Group Leader C3M ballotti@unice.fr<\/a> 0489064332 http:\/\/www.unice.fr\/c3m\/EN\/Equipe1.html<\/a><\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Biology and pathology of melanocytic cells: from cutaneous pigmentation to melanomas<\/strong><\/p>\n
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\n Schematic representation of MITF functions in melanocytes and melanomas<\/td>\n <\/td>\n Our projects are based on very recent and original data obtained in our laboratory, i.e., (i) the identification of a MITF-negative population endowed with melanoma-initiating properties; and (ii) the discovery of a new MITF mutant (E318K) that favours melanoma development.1) We will study the effects of stresses relevant to melanoma development on MITF SUMOylation. We will focus our attention on UV radiation and hypoxia, and then to study the role of MITF SUMOylation, we will compare, in melanoma cells or in mouse model expressing WT or E318K MITF, the effects (migration, invasion, tumorigenicity) of various stimuli identified in the first part of the project to modulate MITF SUMOylation.2) We identified a MITF-negative population, expressing stem cell markers (OCT4, NANOG), displaying high tumorigenic potential and slow growing rate that fits perfectly with the definition of tumor initiating cells. Taking advantage of a new cell surface marker for melanoma initiating cells that we identified recently, we will (i) purify the MIC population (ii) study their biological properties such as growth, migration, differentiation, tumorigenicity, drug resistance and response to environmental stimuli, (iii) Identify the repertoire of gene expressed in the melanoma initiating cells and (iv) validate this marker in human melanoma samples, in correlation the clinical outcome.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Selected Publications<\/strong><\/p>\n
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\n 1.<\/td>\n Major clinical response to a BRAF inhibitor in a patient with a BRAF L597R mutated melanoma Bahadoran, P., Allegra, M., Le Duff, F., Long-Mira, E., Hofman, P., Giacchero, D., Passeron, P., Lacour, JP., Ballotti, R. (2013) J Clin Oncol<\/strong> in press, 1-2<\/td>\n<\/tr>\n \n 2.<\/td>\n A SUMOylation-defective MITF germline mutation predisposes to melanoma and renal carcinoma Bertolotto, C., Lesueur, F., Giuliano, S., Strub, T., de Lichy, M., Bille, K., Dessen, P., d\u2019Hayer, B., Mohamdi, H., Remenieras, A., Maubec, E., de la Fouchardiere, A., Molinie, V., Vabres, P., Dalle, S., Poulalhon, N., Martin-Denavit, T., Thomas, L., Andry-Benzaquen, P., Dupin, N., Boitier, F., Rossi, A., Perrot, J.L., Labeille, B., Robert, C., Escudier, B., Caron, O., Brugieres, L., Saule, S., Gardie, B., Gad, S., Richard, S., Couturier, J., Teh, B.T., Ghiorzo, P., Pastorino, L., Puig, S., Badenas, C., Olsson, H., Ingvar, C., Rouleau, E., Lidereau, R., Bahadoran, P., Vielh, P., Corda, E., Blanche, H., Zelenika, D., Galan, P., Aubin, F., Bachollet, B., Becuwe, C., Berthet, P., Bignon, Y.J., Bonadona, V., Bonafe, J.L., Bonnet-Dupeyron, M.N., Cambazard, F., Chevrant-Breton, J., Coupier, I., Dalac, S., Demange, L., d\u2019Incan, M., Dugast, C., Faivre, L., Vincent-Fetita, L., Gauthier-Villars, M., Gilbert, B., Grange, F., Grob, J.J., Humbert, P., Janin, N., Joly, P., Kerob, D., Lasset, C., Leroux, D., Levang, J., Limacher, J.M., Livideanu, C., Longy, M., Lortholary, A., Stoppa-Lyonnet, D., Mansard, S., Mansuy, L., Marrou, K., Mateus, C., Maugard, C., Meyer, N., Nogues, C., Souteyrand, P., Venat-Bouvet, L., Zattara, H., Chaudru, V., Lenoir, G.M., Lathrop, M., Davidson, I., Avril, M.F., Demenais, F., Ballotti, R*., and Bressac-de Paillerets*, B (2011) Nature<\/strong> 480, 94-98<\/td>\n<\/tr>\n \n 3.<\/td>\n Hypoxia and MITF control metastatic behaviour in mouse and human melanoma cells Cheli, Y., Giuliano, S., Fenouille, N., Allegra, M., Hofman, V., Hofman, P., Bahadoran, P., Lacour, J.P., Tartare-Deckert, S., Bertolotto, C., and Ballotti, R (2012) Oncogene<\/strong> 31, 2461-2470<\/td>\n<\/tr>\n \n 4.<\/td>\n Senescent cells develop a PARP-1 and nuclear factor-{kappa}B-associated secretome (PNAS Ohanna, M., Giuliano, S., Bonet, C., Imbert, V., Hofman, V., Zangari, J., Bille, K., Robert, C., Bressac-de Paillerets, B., Hofman, P., Rocchi, S., Peyron, J.F., Lacour, J.P., Ballotti, R., and Bertolotto, C (2011) Genes & Dev<\/strong> 25, 1245-1261<\/td>\n<\/tr>\n \n 5.<\/td>\n Mitf is the key molecular switch between mouse or human melanoma initiating cells and their differentiated progeny Cheli, Y., Giuliano, S., Botton, T., Rocchi, S., Hofman, V., Hofman, P., Bahadoran, P., Bertolotto, C., and Ballotti, R (2011) Oncogene<\/strong> 30, 2307-2318<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
<\/strong> <\/div>\n<\/li>\n- \n
Barbry Pascal – Physiological Genomics of the Eukaryotes<\/h2>\n
<\/p>\nBARBRY Pascal<\/h3>\n
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\n <\/td>\n <\/td>\n BARBRY Pascal, CNRS<\/strong> Group Leader IPMC direction@ipmc.cnrs.fr<\/a>0493957793 https:\/\/www.ipmc.cnrs.fr\/cgi-bin\/standard.cgi?descriptif=barbry.txt&dossier1=equipes&dossier2=barbry&site=inter&menu=1&ssmenu=2&lang=uk<\/a><\/td>\n <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Physiological Genomics of the Eukaryotes<\/strong><\/p>\n
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\n Immunolabelling of multiciliated airway epithelial cells (RED=basal bodies; GREEN=motille cilia; BLUE=nuclei)<\/td>\n <\/td>\n Respiratory cells develop specific differentiation programs, for instance to synthesize hundreds of motile cilia at the apical surface of the airway epithelial cells. This allows an efficient protection of the respiratory tract mucosa against various external aggressions, and provides specific responses against stresses (allergens, bacteria, viruses, chemicals\u2026). Airway epithelial cells represent a unique cellular model, which is central to important health questions (asthma, lung cancer, cystic fibrosis, fibroproliferative diseases,\u2026). Our group is more specifically interested by the regulations by small regulatory RNAs that can take place in lung cells. In that context, we have recently reported the identification of microRNAs of the miR-34\/miR-449 families as evolutionary conserved key regulators of vertebrate multiciliogenesis. We also found that miR-199a-5p behaves as a major regulator of tissue fibrosis with interesting therapeutic potency to treat fibroproliferative diseases. Our interest for dysregulated microRNAs expression during lung cancer and fibrosis led to investigate the impact of other microRNAs, such as miR-210 and miR-483, on cell metabolism, viability, apoptosis, migration and wound healing. For all these studies, our group develops new approaches in functional genomics and bioinformatics, with a special interest for high throughput sequencing.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Selected Publications<\/strong><\/p>\n
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\n 1.<\/td>\n CDC25A targeting by miR-483-3p decreases CCND-CDK4\/6 assembly and contributes to cell cycle arrest. Bertero T, Gastaldi C, Bourget-Ponzio I, Mari B, Meneguzzi G, Barbry P, Ponzio G, Rezzonico R. (2013) Cell Death Diff<\/strong> 4, e544<\/td>\n<\/tr>\n \n 2.<\/td>\n miR-199a-5p is upregulated during fibrogenic response to tissue injury and mediates TGFbeta-induced lung fibroblast activation by targeting caveolin-1 Cardenas CLL, Henaoui IS, Courcot E, Roderburg C, Cauffiez C, Aubert S, Copin MC, Wallaert B, Glowacki F, Dewaeles E, Milosevic J, Maurizio J, Tedrow J, Marcet B, Lo-guidice JM, Kaminski N, Barbry P, Luedde T, Perrais M, Mari B, Pottier N. (2013) PLoS Genet<\/strong> 9(2), e1003291<\/td>\n<\/tr>\n \n 3.<\/td>\n Gene expression profiling reveals distinct epithelial phenotypes in child respiratory allergy Giovannini-Chami L, Marcet B, Moreilhon C, Chevalier B, Illie MI, LeBrigand K, Robbe-Sermesant K, Bourrier T, Michiels JF, Mari B, Cr\u00e9nesse D, Hofman P, de Blic J, Castillo L, Albertini M, Barbry P. (2012) Eur Resp J<\/strong> 39(5), 1197-205<\/td>\n<\/tr>\n \n 4.<\/td>\n Small RNA sequencing reveals miR-642a-3p as a novel adipocyte-specific microRNA and miR-30 as a key regulator of human adipogenesis. Zaragosi LE, Wdziekonski B, Le Brigand K, Villageois P, Mari B, Waldmann R, Dani C, Barbry P. (2011) Genome Biol<\/strong> 12(7) , R64<\/td>\n<\/tr>\n \n 5.<\/td>\n miR-449 microRNAs trigger vertebrate multiciliogenesis through direct repression of the Notch ligand Delta-like 1. Marcet B, Coraux C, Chevalier B, Luxardi G, Zaragosi LE, Robbe-Sermesant K, Jolly T, Cardinaud B, Moreilhon C, Giovannini-Chami L, Birembaut P, Waldmann R, Kodjabachian L, Barbry P. (2011) Nature Cell Biology. <\/strong>13(6), 694-701<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Awards<\/strong> <\/strong> 2011: CNRS PES award 2010: Coordinator of InDiGen, a component of France-G\u00e9nomique <\/div>\n<\/li>\n
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Bardoni Barbara – Physiopathology of intellectual disability<\/h2>\n
<\/p>\nBARDONI Barbara<\/h3>\n
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\n <\/td>\n <\/td>\n BARDONI Barbara, Inserm<\/strong> Group Leader IPMC bardoni@ipmc.cnrs.fr<\/a> 0493957766\/62\/78 https:\/\/www.ipmc.cnrs.fr\/cgi-bin\/standard.cgi?descriptif=bardon<\/a><\/td>\n <\/td>\n <\/td>\n <\/td>\n <\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Physiopathology of Intellectual disability<\/strong><\/p>\n
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\nCopyright : Arkowitz, Avella, Braendle, Delaunay, Descombes, Fonta, G\u00e9minard, Plourabou\u00e9, Slagmulder, Van de Bor.<\/div>\nPlease note that all Team Presentation Forms below have been updated in the 2015 Pamphlet :<\/span> here<\/a><\/strong><\/p>\n
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